MasterTech Special Stain Kits

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  1. ONESTEP TRICHROME BLU/RED GL KT/Ea
    Item # : KTTRBGAL
    Stains for collagen and muscle in one hour and 15 minutes.

    The MasterTech One-Step Trichrome Kit makes staining for collagen or muscle, fast and easy. Unlike traditional one-step procedures, Modified Mayer's Hematoxylin is used instead of Weigerts Hematoxylin, eliminating the need for unnecessary steps, while making this kit ready-to-use. One Step Trichrome serves as the primary stain, producing pink to red cytoplasm, intercellular fiber, keratin, and muscle, blue or green collagen, and mucus, depending on the Trichrome color chosen, and blue nuclei stained by Mayer’s Hematoxylin.
  2. Mod Brown Hopps Gram PT Stain Kt/Ea
    Item # : KTBHGPT
    Stains for gram positive and gram negative bacteria in just under 20 minutes.

    This kit is suitable in lieu of and comparable to various Brown-Hopps staining procedures. Basic Fuchsin and Crystal Violet both serve as primary stains, producing blue stained gram positive bacteria, along with red gram negative bacteria and nuclei, while the Picric Acid/Acetone provides a yellow counterstain.
  3. IRON LITER STAIN KIT /Ea
    Item # : KTIROLT
    Stains ferric iron in under 30 minutes.

    This kit is suitable in lieu of and comparable to the Perl’s Technique and Gomori’s Iron Reaction for staining ferric iron. The MasterTech Iron Stain is less hazardous than these procedures and provides more precise results by using lower strength Hydrochloric Acid and Potassium Ferrocyanide. A Working Prussian Blue Solution serves as the primary stain, producing blue ferric iron against a pink to red background with red nuclei, counterstained by Nuclear Fast Red. There is no filtration required when using MasterTech Nuclear Fast Red.
  4. STEINER STAIN KIT /Ea
    Item # : KTSTE
    Stains for Donovan bodies, fungi, Helicobacter pylori, Legionella pneumophila, and spirochetes in 45 minutes.

    This kit includes a 45 minute microwave procedure and the traditional one hour procedure. This kit is suitable in lieu of and comparable to the Traditional Steiner and Dieterle’s stain. The MasterTech Steiner Stain Kit differs from simliar kits by eliminating radioactive Uranyl Nitrate, providing pre-measured Hydroquinone capsules, and modifying the original technique, which creates a safer, easier to use procedure that provides optimal staining results and reduced background staining. Silver Nitrate, developed by the Hydroquinone based reducing solution serves as the primary stain, producing dark brown to black donovan bodies, fungi, Helicobacter pylori, legionella pneumophila, and spirochetes, against a yellow to brown background.
  5. ONESTEP TRICHROME GRN /RD PT KIT/Ea
    Item # : KTTRGPT
    Stains for collagen and muscle in one hour and 15 minutes.

    The MasterTech One-Step Trichrome Kit makes staining for collagen or muscle, fast and easy. Unlike traditional one-step procedures, Modified Mayer's Hematoxylin is used instead of Weigerts Hematoxylin, eliminating the need for unnecessary steps, while making this kit ready-to-use. One Step Trichrome serves as the primary stain, producing pink to red cytoplasm, intercellular fiber, keratin, and muscle, blue or green collagen, and mucus, depending on the Trichrome color chosen, and blue nuclei stained by Mayer’s Hematoxylin.
  6. CHANDLER'S RETICULUM LITER KIT/ Ea
    Item # : KTCPRLT
    Stains reticulum fibers with no background staining in 30 minutes.

    This reticulin kit is suitable in lieu of and comparable to the Gomori, Gordon and Sweets, Snook’s, Laidlaw’s, Wilder’s, Modified Gridley’s, and Foot’s technique staining for Reticular Fibers, often performed during the differential diagnosis of various types of tumors or liver diease. In addition to being much shorter than many of these procedures, the MasterTech Chandler’s Precision® Reticulum Stain Kit does not use radioactive Uranyl Nitrate, or cumbersome Lithium Silver solutions. With easy to use, pre-measured Silver Solution vials and a specially formulated Nuclear Fast Red that reduces flocculation, this kit will produce vibrant nuclei and contrasting reticulum fibers with a background free of staining artifacts and debris. An Ammoniacal Silver Developer Solution serves as the primary stain, producing brown to black reticulum fibers against a pink to red background counterstained by Nuclear Fast Red. There is no filtration required when using MasterTech Nuclear Fast Red.
  7. HEPATITIS B STAIN KIT /Ea
    Item # : KTHEP
    Stains for Hepatitis B surface antigen (HBsAg) in two hours and thirty minutes.

    Orcein Stain serves as the primary stain, differentiated to produce red to brown aggregates in the cytoplasm of hepatocytes and Kupffer cells against a yellowish brown background.
  8. A.F.B. STAIN KIT /Ea
    Item # : KTAFB
    Stains for mycobacteria (acid fast bacteria) in less than 15 minutes.

    This kit is suitable in lieu of and comparable to the Kinyoun’s AFB and Ziehl-Neelsen procedures for staining all mycobacteria, including Mycobacterium tuberculosis. The MasterTech AFB Stain Kit differs from these procedures in that Advanced Carbol Fuchsin is used for bacterial staining instead of a standard Carbol Fuchsin, and Light Green is used as a counterstain instead of Methylene Blue. In comparison to standard Carbol Fuchsins, MasterTech ACF™ requires no heat and much shorter staining times by combining all three fuchsin dyes. Light Green is a much better counterstain than Methylene Blue, as it provides higher contrast against the red stained bacteria.
  9. P.T.A.H. STAIN KIT /Ea
    Item # : KTPTA
    Stains for collagen, fibrin, and striated muscle in under one hour.

    This kit includes a 55 minute microwave procedure and the traditional 15 hour procedure. Unlike similar kits on the market, the MasterTech P.T.A.H. Stain Kit is less hazardous, using Zinc Chloride instead of mercuric based mordants, which also eliminates the need for Potassium Dichromate. Ferric Ammonium Sulfate is also used instead of Acidic Potassium Permanganate, further reducing the stain kit’s hazard. P.T.A.H. serves as the primary stain, producing blue fibrin, muscle, and nuclei, with brown to red collagen.
  10. MOD BROWN & BRENN PINT STAIN KIT/Ea
    Item # : KTBBRPT
    Stains for gram positive and gram negative bacteria in just 10 minutes.

    The MasterTech Brown and Brenn Stain Kit is less hazardous than similar kits due to a specially formulated Advanced Picric Acid/Acetone. Safranin O and Hucker’s Gentian Violet both serve as primary stains, producing blue stained gram positive bacteria, along with red gram negative bacteria and nuclei, while the Advanced Picric Acid/Acetone provides a yellow counterstain.
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