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Stains for the two types of muscle fibers and their subtypes in 50 minutes.
Traditional ATPase procedures employ the use of a barbiturate buffer, which is a controlled substance. American MasterTech’s Myofibrillar ATPase Stain Kit contains no barbiturates! In the MasterTech’s Myofibrillar ATPase Stain Kit a predominance of Type 1 fiber types typically indicates a myopathic condition, such as dystrophy, while a predominance of Type 2 fibers typically indicates a neuropathic condition. Type 1 Buffer stains small type 1 fibers black and type 2C fibers grey. Type 1&2BC Buffer stains small type 1 fibers black, 2B and 2C fibers grey to black. Type 2AB Buffer stains type 2A and 2B fibers black.
Stains for the two types of muscle fibers and their subtypes in 50 minutes. Traditional ATPase procedures employ the use of a barbiturate buffer, which is a controlled substance. American MasterTech’s Myofibrillar ATPase Stain Kit contains no barbiturates! In the MasterTech’s Myofibrillar ATPase Stain Kit a predominance of Type 1 fiber types typically indicates a myopathic condition, such as dystrophy, while a predominance of Type 2 fibers typically indicates a neuropathic condition. Type 1 Buffer stains small type 1 fibers black and type 2C fibers grey. Type 1&2BC Buffer stains small type 1 fibers black, 2B and 2C fibers grey to black. Type 2AB Buffer stains type 2A and 2B fibers black. 100ml Stain Kit yields between 36 and 45 slides. Pint Stain Kit yields between 144-225 slides. Liter Stain Kit yields between 306-450 slides. Slide Yields assume maximum slides stained per run, maximum runs per use. Actual Slide Yields may vary.
100ml Kit Consists of: Type 1 Buffer 100ml, Type 1 &2BC Buffer 100ml, Alkaline Buffer Stock 100ml x 2, Calcium Chloride Solution 100ml, Cobalt Chloride Solution 100ml, 1N Sodium Hydroxide 15ml, 1N Hydrochloric Acid 15ml, ATP Powder 200mg.
Pint Kit Consists of: Type 1 Buffer Pint, Type 1 &2BC Buffer Pint, Alkaline Buffer Stock Pint x 2, Calcium Chloride Solution Pint, Cobalt Chloride Solution Pint, 1N Sodium Hydroxide 50ml, 1N Hydrochloric Acid 50ml, ATP Powder 1,000mg.
Liter Kit Consists of: Type 1 Buffer Liter, Type 1 &2BC Buffer Liter, Alkaline Buffer Stock Liter x 2, Calcium Chloride Solution Liter, Cobalt Chloride Solution Liter, 1N Sodium Hydroxide 100ml, 1N Hydrochloric Acid 100ml, ATP Powder 2,000mg.
All kit components are available for purchase individually. Both kits are sealed with a tamper-evident label for quality assurance. All kit components should be stored in a dry environment between 15º C and 30º C. Please review all component labels to ensure correct storage temperature.